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Identification of PCFT mutant clones with impaired transport function. MTX influx was assessed at 0.5 μm and pH 5.5 over 1 min in HeLa R1–11 cell transient transfectants expressing mutant and wild-type PCFTs. The data are presented as percentage of wild-type PCFT activity. p values are derived from paired t tests comparing mutants and mock transfectants. Data are the mean ± S.E. from three independent experiments.
