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Concentration dependence of the PCFT mutant transport function. MTX influx was performed in transient transfectants under standard conditions (pH 5.5 and over 1 min) at concentrations of 0.5 and 50 μm. MTX influx is expressed in units of picomole/mg of protein (panel A) or as a percentage of wild-type PCFT activity (panel B) at each substrate concentration. Data are the mean ± S.E. from three independent experiments.
