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. 2011 May 3;286(27):24324–24335. doi: 10.1074/jbc.M110.220814

FIGURE 2.

FIGURE 2.

GABAB receptors are internalized via clathrin and dynamin-dependent mechanisms and recruited to lysosomes. Shown are the rate (A) and extent (B) of constitutive internalization of BTX-AF555-tagged R1aBBSR2 receptors in the absence (●) and presence of dynasore (▴) or chlorpromazine (□). GIRK cells expressing R1aBBSR2 receptors were incubated in 3 μg/ml BTX-AF555 for 10 min at room temperature and imaged over 0–60 min at 30–32 °C in the presence of either 50 μg/ml CPZ or 80 μm DYN, n = 5–11. **, p < 0.05 (one-way ANOVA). C, hippocampal neurons (14–21 DIV) expressing R1aBBSR2 and either eGFP-Rab5, eGFP-Rab11, or Rab7-eGFP were incubated in 1 mm d-tubocurarine for 5 min, followed by 3 μg/ml BTX-AF555 for 10 min at room temperature. Cells were incubated at 37 °C for 30–60 min and then fixed and imaged. The arrowheads depict co-localization in the soma (top) and a dendrite (bottom). Scale bars, 5 μm; error bars, S.E.