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Differential binding of SRCL to milk and neutrophil lactoferrin. A, SDS-polyacrylamide gels of lactoferrin were stained with Coomassie Blue or blotted onto nitrocellulose and probed with SRCL conjugated to alkaline phosphatase (SRCL-AP). B, comparison of fractionation of neutrophil and milk lactoferrin on immobilized SRCL. Fractions from the affinity column were analyzed on SDS-polyacrylamide gels that were stained with Coomassie Blue.
