FIGURE 9.
Internalization of glycoproteins by transfected fibroblasts expressing SRCL. A, neoglycoprotein LNFPIII-BSA and control BSA ligands. B, neutrophil lactoferrin and ovalbumin ligands. Cells were incubated for the indicated times at 37 °C with fluorescein-labeled glycoproteins. After fixation, bound and internalized glycoproteins were visualized with antibodies to fluorescein followed by Alexa 488-labeled secondary antibodies. Nuclei were visualized with DAPI stain. Bar, 20 μm.