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. 2011 May 11;286(27):24336–24349. doi: 10.1074/jbc.M111.244772

FIGURE 9.

FIGURE 9.

Internalization of glycoproteins by transfected fibroblasts expressing SRCL. A, neoglycoprotein LNFPIII-BSA and control BSA ligands. B, neutrophil lactoferrin and ovalbumin ligands. Cells were incubated for the indicated times at 37 °C with fluorescein-labeled glycoproteins. After fixation, bound and internalized glycoproteins were visualized with antibodies to fluorescein followed by Alexa 488-labeled secondary antibodies. Nuclei were visualized with DAPI stain. Bar, 20 μm.