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Production of HxcT variants. 15% SDS-PAGE and Western blot of cell extracts from the ΔxcpTΔhxcT strain producing XcpT, HxcT, and the various HxcT variants and grown on Luria broth plate supplemented with 2 mm isopropyl β-d-1-thiogalactopyranoside. The membrane was revealed by anti-XcpT (left) or anti-HxcT (right) antibodies. An arrow indicates the position of the proteins. Molecular mass markers are indicated on the left side of each panel.
