FIGURE 5.

All HxcT variants remain functional in the Hxc secreton. Analysis of the supernatant of various P. aeruginosa strains grown under phosphate-limitation conditions is shown. The equivalent of 1.0 A₆₀₀ has been loaded in each well. Samples were first run on a 12% SDS-PAGE and then blotted and revealed using an antibody directed against LapA. Two strains of P. aeruginosa were used here: the PAO1 parental strain and its D40ZQΔhxcT derivative, which lacks the entire xcp T2SS operon together with an in-frame deletion of the hxcT gene. The two strains were complemented either with the empty vector or with plasmids expressing the wild-type hxcT gene or its mutant derivatives. The position of LapA is indicated by an arrow on the right side of the gel.