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. 2011 May 19;286(27):24443–24450. doi: 10.1074/jbc.M110.197525

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — The G3036E Cbl mutant marginally modulates hMSC proliferation and survival. A and B, quantitative PCR analysis (A) and Western blot analysis (B) showing increased Cbl mRNA and protein levels, respectively, in clonal hMSCs transduced with the G306E Cbl mutant compared with the empty vector (EV). C and D, effect of the G3036E Cbl mutant on hMSC cell replication as shown by BrdU assay (C) and cell number (D). E and F, change in cell survival induced by the G3036E Cbl mutant in clonal hMSCs as shown by MTT assay (E) and TUNEL staining in serum-deprived conditions (F). *, significant difference with EV-transduced cells (p < 0.05). Error bars, S.D.