FIGURE 7.

NMP suppresses RANKL-induced NFATc1 expression. RAW264.7 cells were stimulated with RANKL alone or with different concentrations of NMP as indicated (48 h for mRNA and 72 h for proteins). a, total RNA was extracted, and NFATc1 mRNA was determined by real-time PCR as described under “Experimental Procedures.” Data are expressed as the mean ± S.D. (n = 3) from a representative experiment. b, whole cells were subjected to Western blot analysis with antibody against NFATc1. Anti-actin antibody was used to visualize the loading control. Rel Quant, relative quantity.