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The biotinylated and unlabeled capture probes used to detect Sp1 binding activity in the NoShift Assay
Putative recognition sequences are underlined.
| Oligoucleotides | Sequence (5′ to 3′) |
|---|---|
| −253 | GCCGCTCGCGGTAGGGGCGGAGCGCTGAGCTGG |
| −276 | CGCCACGCACGCAGCCCCACCCAGCGCCGCTCGC |
| −312 | CTCCTACACCTGCGGCGCGCCCCCGTCCCCTGGTCCCAG |
| −490 | TCCGGTCGCCTCGGCCCACCACCC |
| −515 | GACCGGAAAGGCGGCCAGGACCTT |
| −548 | GAAACCCGAGGGCGGCGCTCCAGG |
| Sp1-consensus | CCCTTGGTGGGGGCGGGGCCTAAGCTGCG |
