Oligonucleotide sequences containing six Sp1 sites harbored in the human UGDH gene promoter
Mutations were introduced into Fragment 3 using QuikChange site-directed mutagenesis kit (Stratagene) and fused to a reporter construct. The resulting constructs underwent confirmatory sequencing. Mutated nucleotides are italicized. Wild type sequences appear above mutated bases. Putative recognition sequences are underlined.