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Dose-response curve. (A) Representative trace depicting the fluorescence resonance energy transfer (FRET) ratio changes (yellow fluorescent protein:monomeric Turquoise (YFP:mTq)) upon addition of increasing amounts of histamine (gray bars) in HeLa cells expressing Gαq-mTqΔ6, Gβ1, YFP-Gγ2 and the histamine H1 receptor (H1R). (B) The mTq and YFP intensity traces from which the ratio was derived.
