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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Neurochem. 2011 Jun 13;118(2):215–223. doi: 10.1111/j.1471-4159.2011.07319.x

Figure 3. Mouse MCP1 C-terminus inhibits the formation of lamellipodia induced by human MCP1.

Figure 3

A. Primary microglia plated on coverslips were incubated with a point source of recombinant MCP1 proteins for different periods of time. At each time point, the cells were washed, fixed, and stained for actin cytoskeleton using Alexa-Phalloidin. Scare bar = 10 μm. The percentage of unipolar cells (migrating towards the focal point of MCP1 protein) was quantified. Data are expressed as Mean ± SD. N=9. *p<0.05. B. Mouse MCP1 C-terminus changes the activation pattern of ERK on microglia. Primary microglial cells were treated with h-or hc-MCP1 over time. At each time point, cell lysates were collected and analyzed for phosphorylated and total ERK1/2 by western blotting. Experiments were performed in triplicate. Representative experiment shown. Data are expressed as Mean ± SD. *p<0.05, compared to 0 time point.