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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Nat Cell Biol. 2011 Jun 5;13(7):790–798. doi: 10.1038/ncb2268

Figure 3.

Figure 3

Tubulin point mutants are temperature sensitive. (a) Wild type and the two deletion mutants, tbb-4(OK1461) and tba-5(tm4200), were nearly 100% stained in the amphid and phasmid neurons at 15°C, 20°C and 25°C. tbb-4(sa127) and tba-5(qj14) worms were not stained when grown at 15°C. But at 25°C, around 50% of the amphids and 20% phasmids are stained while tba-5(dyf-10) has very little temperature effect. n indicates the number of amphids or phasmids. (b) Visualized with a TBB-4::YFP tubulin marker, tba-5(qj14) possessed only the middle segment of the amphid and phasmid cilia at 15°C, but full-length cilia could be seen in tba-5(qj14) at 25°C. Bar=5 μm. Arrows point to transition zones with cilia oriented upward. (c) Visualized with an OSM-6::GFP marker, tbb-4(sa127) possessed only the middle segment of the amphid and phasmid cilia at 15°C, but full-length cilia could be seen in tbb-4(sa127) at 25°C. Bar=5 μm. Arrows point to transition zones with cilia oriented upward. The wild type image of the TBB-4::YFP marker in b and c was the same as used in Fig. 1e and Fig. S3b.

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