Figure 2. Transcriptional regulation of HER2 mediated IL-6 expression.
A) Kinase inhibition (10 μM) of IL-6 secretion in MCF-10a-HER2 cells (N=3, 24 hours post-treatment, bars represent SD). B and C) NF-kB, AP-1, and C/EBP activity in MCF-10a and MCF-10a-HER2 cells (B) or NIH/3T3 or NIH/3T3-HER2 cells (C). In all samples, luciferase expression measured at 24 hours post-transfection (hpt), N=4, bars represent SD. D) NF-kB inhibition of IL-6 secretion in MCF-10a-HER2 cells (N=4, 24hpt, bars represent SD). Where indicated * and ** represent p<.05 and p<.01 respectively in comparison to controls. E) Schematic diagram of the signaling pathways regulating HER2-induced transcriptional upregulation of IL-6.