Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Mol Carcinog. 2011 Mar 22;50(8):614–624. doi: 10.1002/mc.20760

Figure 1.

Figure 1

p53 tumor suppressor was largely dispensable for WA-induced apoptosis. (A) Chemical structure of WA. (B) Western blotting for p53, S15 phosphorylated p53, and full PARP protein using lysates from MCF-7 cells transiently transfected with a nonspecific siRNA or a p53-targeted siRNA and treated for 24 h with DMSO (control) or 2.5 μM WA. Numbers above bands indicate change in protein levels relative to DMSO-treated-nonspecific siRNA-transfected MCF-7 cells. (C) Histone-associated DNA fragment release into the cytosol in MCF-7 cells transiently transfected with a nonspecific siRNA or a p53-targeted siRNA and treated for 24 h with DMSO (control) or 2.5 μM WA. Results shown are mean ± SD (n= 3). Significantly different (P < 0.05) (a) compared with corresponding DMSO-treated control, and (b) between WA-treated nonspecific siRNA transfected and WA-treated p53 siRNA transfected cells by one-way ANOVA followed by Bonferroni's test.