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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Mol Carcinog. 2011 Mar 22;50(8):614–624. doi: 10.1002/mc.20760

Figure 2.

Figure 2

WA-induced apoptosis was attenuated in the presence of 17β-estradiol (E2). (A) Trypan blue dye exclusion assay for effects of WA and/or E2 treatments (24 h treatment) on viability of MCF-7 cells. Live cells were counted and normalized as 100% to DMSO-treated cells in the absence of E2. (B) Histone-associated DNA fragment release into the cytosol in MCF-7 cells treated for 24 h with WA and/or E2. Results shown are expressed as enrichment factor relative to DMSO-treated control cells (without WA or E2 treatment). (C) Immunodetection of full PARP protein using lysates from MCF-7 cells treated for 24 h with WA in the absence or presence of E2. Results shown are mean ± SD (n= 3). Significantly different (P < 0.05) (a) compared with DMSO-treated control and (b) between without and with E2 treatment groups by one-way ANOVA followed by Bonferroni's test.