Figure 1.

Defective CHS response to DNFB in TMA-treated ER-TAK1^fl/fl mice. (a) On day 1, ER-TAK1^fl/fl mice were painted with DNFB mixed with (+) or without (−) TMA on their dehaired back skin. Mice were challenged with DNFB on the left ear skin on day 6. Ear thickness was measured before (open bar) and 48 h after (solid bar) the challenge and plotted (lower left panel). The changes in ear thickness (Δ ear thickness) were also calculated and plotted (lower right panel). Mean±SD values of four mice are shown, and the P value is indicated. (b) ER-TAK1^fl/fl mice were painted with TMA (+) or not (−). The painted skin were excised and subjected to PCR analysis for TAK-deleted alleles (ΔTAK1) and Cre (served as internal control). (c) A CHS response was induced in indicated mouse strains with or without TMA as shown in the scheme (left panel). The ear thickness was measured before and after challenge as described in (a), and Δ ear thickness was calculated and plotted (right panel). Mean±SD values of three mice are shown, and the P value is indicated. CHS, contact hypersensitivity; DNFB, 2,4-dinitrofluorobenzene; TAK1, transforming growth factor-β-activated kinase-1; TMA, tamoxifen.