Figure 3.

Lack of activation and cytokine production of CD4 and CD8 T cells in TMA-treated ER-TAK1^fl/fl mice. Flow cytometry analysis of CD62L and CD44 expression on CD4 (a) and CD8 (b) T cells in DLN from indicated mice 48 h after challenge (left panels). The percentages of the CD62L^low CD44^high effector T-cell population were plotted (right panels). Mean±SD values of four mice are shown, and the P value is indicated. (c) IFN-γ and IL-17 production by CD8 cells in the DLN of indicated mice (left panels). The frequencies (%) of IFN-γ-producing CD8 cells obtained from four independent experiments are shown. Mean±SD values are shown and the P value is indicated. (d) IFN-γ, IL-4 and IL-17 production in CD4 cells in DLN from indicated mice were detected by intracellular staining. Representative results of four independent experiments are shown. CLN, control lymph node; DLN, draining lymph node; IFN, interferon; TAK1, transforming growth factor-β-activated kinase-1; TMA, tamoxifen.