Figure 4.

Abolished CHS response in CD11c-TAK1^fl/fl mice. (a) On day 1, the dehaired back skins of CD11c-TAK1^fl/fl and CD11c-TAK1^fl/wt mice were painted with DNFB. Mice were then challenged with DNFB on day 6. Before (open bar) and 48 h after (solid bar) challenge, ear thickness was measured and plotted (top panel). The Δ ear thickness was also calculated and plotted (lower panel). Mean±SD values of four mice are shown, and the P value is indicated. (b) CD11c⁺ and CD4⁺ cells were purified from CD11c-TAK1^fl/wt mice. PCR analysis for the floxed-TAK allele (TAK1^fl) and WT TAK1 allele (TAK1^wt) (which served as an internal control) was performed. CHS, contact hypersensitivity; DNFB, 2,4-dinitrofluorobenzene; TAK1, transforming growth factor-β-activated kinase-1; WT, wild type.