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. Author manuscript; available in PMC: 2012 Jul 1.

Published in final edited form as: J Thromb Haemost. 2011 Jul;9(7):1308–1317. doi: 10.1111/j.1538-7836.2011.04331.x

APC activates AMPK phosphorylation in isolated mice cardiomyocytes. (A) Immunoblotting for EPCR in mice heart or cardiomyocytes. (B) APC stimulates the phosphorylation of AMPK in cardiomyocytes by a concentration- and (C) time-dependent manner. (D) The phosphorylation of AMPK in isolated mouse cardiomyocytes treated with different APC derivatives. Activation of AMPKα1 (E) and AMPKα2 (F) by different APC derivatives in isolated mouse cardiomyocytes. Values are means ± S.E., n=4. *p<0.05 vs. control. (G) AMPK activation by APC and APC-2Cys in isolated cardiomyocytes with or without pre-incubation with function-blocking anti-EPCR (RCR252) and anti-PAR-1 (H-111) antibodies. Values are means ± S.E., n=4. *p<0.01 vs. control; †p<0.05 vs. APC alone; ^#p<0.05 vs. APC-2Cys alone.

APC activates AMPK phosphorylation in isolated mice cardiomyocytes. (A) Immunoblotting for EPCR in mice heart or cardiomyocytes. (B) APC stimulates the phosphorylation of AMPK in cardiomyocytes by a concentration- and (C) time-dependent manner. (D) The phosphorylation of AMPK in isolated mouse cardiomyocytes treated with different APC derivatives. Activation of AMPKα1 (E) and AMPKα2 (F) by different APC derivatives in isolated mouse cardiomyocytes. Values are means ± S.E., n=4. *p<0.05 vs. control. (G) AMPK activation by APC and APC-2Cys in isolated cardiomyocytes with or without pre-incubation with function-blocking anti-EPCR (RCR252) and anti-PAR-1 (H-111) antibodies. Values are means ± S.E., n=4. *p<0.01 vs. control; †p<0.05 vs. APC alone; ^#p<0.05 vs. APC-2Cys alone.