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Hydrodynamic diameter (z-average) of CMVL/DOPC–DNA complexes at ΦCMVL = 0.6 in Opti-MEM 20 minutes after complex formation. The samples were prepared at values of L/D optimal for transfection (L/D = 10.0 for CMVL5, L/D = 10.7 for CMVL4, L/D = 20.2 for CMVL3, and L/D = 13.7 for CMVL2, see below). Data points shown are the average of duplicate measurements of the same sample, with error bars showing the standard deviation.
