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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: J Neurochem. 2011 Jun 6;118(2):237–247. doi: 10.1111/j.1471-4159.2011.07308.x

Figure 4.

Figure 4

Viral mediated expression of GluR1(S845A) in cultured medium spiny neurons blocks SKF81297-evoked phosphorylation of GluR1 and glutamate-evoked currents. (A) Photomicrographs of neurons that were (left) not infected, (middle) infected with Lenti-GFP, or (right) infected with Lenti-GluR1(S845A). Scale bar = 20μm. (B) Representative immunoblots of pGluR1(S845) and GluR1, (C) pGluR1(S845) (***, P<0.001, vs remaining groups; Scheffé), and (D) GluR1 protein levels in the three groups of neurons either incubated or not incubated with SKF81297. n/group=4-7. (E) Representative current traces obtained in the three groups of neurons before and after focal application of glutamate (arrowheads). (F) Mean peak currents obtained following glutamate in the three groups of neurons (**, P<0.01, vs Control non-infected cells; Scheffé). n/group=5-12. Data are shown as mean±SEM.