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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Gastroenterology. 2011 Apr 2;141(1):310–319. doi: 10.1053/j.gastro.2011.03.056

Fig. 6. Characterization of EFS-induced relaxation.

Fig. 6

In order to characterize the chemical nature of inhibitory neurotransmission, bioengineered intrinsically innervated IAS constructs were pre-incubated with inhibitors before stimulation with electrical field. The inhibitors blocked nitric oxide synthesis (L-NAME) or antagonized VIP receptors (VIP-Ra). In the presence of L-NAME or VIP-Ra, the magnitude of EFS-induced relaxation was significantly reduced (P<.05). This implies involvement of both nitrergic and VIP-ergic inhibitory stimulation in response to electrical field. TTX abolished EFS-induced relaxation, indicating that the process was entirely neuronally mediated. Values are mean ± SEM of 3 experiments.