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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Steroids. 2011 Feb 1;76(9):921–928. doi: 10.1016/j.steroids.2011.01.008

Fig. 1.

Fig. 1

C terminus PR primer design and RT-PCR detection of C terminus PR mRNA expression in breast cancer cell lines. A, Schematic diagram of encoded protein showing DNA binding domain (DBD) and ligand binding domain (LBD). Two pairs of primers were designed within the range of LBD. S1 and S2, sense primer 1 and 2; AS1 and AS2, antisense primer 1 and 2. B, RT-PCR results. M: DNA size makers; T47D: PR positive T47D Yb cells; 231: MDA-MB-231 cells; 231-mPRα: human mPRα transfected MDA-MB-231 cells; 231-mPRβ: human mPRβ transfected MDA-MB-231 cells; 468: MDA-MB-468 cells.