Figure 1.

Vector map of pREV and anti-RAD51 ribozyme/target mRNA sequences. (A) The plasmid vector pREV used to insert REOs for expression of ribozymes in mammalian cells. When the RAD51 REO was inserted into pREV, the vector was denoted pREVRz. The gene for EYFP was ligated into the REO insertion site of pREV (called pREVY). (B) RAD51 ribozyme bound to its target mRNA sequence. The top row of nucleotides shows the target molecule with the GUC target site underlined. Bold numbers show residue numbering of the mRNA sequence. The ribozyme is shown underneath and bold letters represent the catalytic core region (16). The arrow indicates the site of cleavage.