Fig. 1.

Characterization of the rtl1 mutant. a Kinetics of the fresh weight change in the detached rosette leaves from 4-week-old wild-type (WT) (open circles) and rtl1 (closed circles) plants. The relative weights of leaves are presented as a percentage of the initial weight, which was the weight of each rosette leaf immediately after detachment from the plants, with the standard deviation (SD). b Four-week-old WT and rtl1 plants. Bar 10 mm. c Effect of ABA on light-induced stomatal opening in WT and rtl1 plants. Epidermal tissues from dark-adapted plants (shaded) were incubated under light (blue light at 10 μmol m⁻² s⁻¹ superimposed on background red light at 50 μmol m⁻² s⁻¹) for 2.5 h with (black) or without (white) 20 μM ABA. Data represent the mean with SD. d ABA-induced stomatal closing in WT and rtl1 plants. Pre-illuminated epidermal tissues were incubated under light (same as above) for 2.5 h with (black) or without (white) 20 μM ABA. Data represent the mean with SD. All experiments were repeated three times on different occasions with similar results