Fig. 3.

Phenotypes in wild-type (WT), rtl1, and CHLH RNAi lines. a RT-PCR analysis of CHLH in WT, rtl1, and two CHLH RNAi lines (#1 and #2). Total RNA was isolated from rosette leaves from 4-week-old plants. PCRs were performed with 26 cycles. TUB2 was amplified as a control. b Immunoblot analysis of CHLH protein in WT, rtl1, and CHLH RNAi lines. Fifty micrograms of protein extracted from rosette leaves was loaded on each lane. The 14-3-3 proteins were detected using anti-14-3-3 antibody as a control. c Four-week-old WT, rtl1, and CHLH RNAi plants. Bar 10 mm. d Chlorophyll content of rosette leaves from 4-week-old WT, rtl1, and CHLH RNAi plants. The bars show the content of chl a (white) and chl b (black). e Effect of ABA on stomatal closing in WT, rtl1, and CHLH RNAi plants. Conditions were the same as in Fig. 1d. Data represent the mean with SD. All experiments repeated three times on different occasions gave similar results