Table 1.
Burkholderia pseudomallei strains used in this study.
| Strain | Description | Source |
|---|---|---|
| Strain 1026b-based mutants | ||
| 1026b | Clinical isolate, wild-type | DeShazer et al. (1997) |
| Bp319 | 1026b ΔpenA | This study |
| Bp409 | 1026b ΔtatABC | This study |
| Bp420 | 1026b ΔpenA ΔtatABC | This study |
| Bp343 | 1026b ΔBPSS0945 | This study |
| Bp344 | 1026b ΔBPSS0944 | This study |
| Bp349 | 1026b ΔBPSS0948 | This study |
| Bp342 | 1026b PenA R7K | This study |
| Bp421 | 1026b PenA R8A | This study |
| Strain Bp82-based mutants | ||
| Bp82 | 1026b ΔpurM | Propst et al. (2010) |
| Bp82.3 | Bp82 PenA C69Y | This study |
| Bp82.4 | Bp82 PenA S72F | This study |
| Bp82.5 | Bp82 PenA P167S | This study |
| Bp82.11 | Bp82 ΔpenA | This study |
| Bp82.14 | Kmr; Bp82:Tn7T-Ps12-FKM-lox-BPSS0944a | This study |
| Bp82.15 | Kmr; Bp82:Tn7T-Ps12-FKM-lox-BPSS0945 | This study |
| Bp82.16 | Kmr; Bp82:Tn7T-Ps12-FKM-lox-BPSS0948 | This study |
| Bp82.21 | Kmr; Bp82:Tn7T-LAC-FKM-penA + b | This study |
aCloned genes are transcribed from the constitutive B. thailandensis ribosomal s12 gene promoter.
bCloned penA gene is transcribed from the IPTG-inducible E. coli lactose operon/tryptophan hybrid promoter Ptac.