Table 3.
Primers used in this study.
| Primer | Sequencea,b,c,d | Source |
|---|---|---|
| CLONING/DELETION | ||
| 1687 | 5′-GGATCCGACGAGAGCTGATACGCTAG | This study |
| 1712 | 5′-AAGCTTATACCGGCATCGTTTCGCTG | This study |
| 2003 | 5′- GAATTCGATACCGGCATCGTTTCG | This study |
| 2004 | 5′-GATATCAGCCGTTGACTTAGTTGGTATTTCCGGAATATCATGCTGGTTCCGAATAA TTTTGTTTAACTTTAAGAAGGAGATATAC | This study |
| 2005 | 5′-ACTTTAAGAAGGAGATATACATGAATCATTCTCCGTTGCGC | This study |
| 2006 | 5′-CAATCTCGACGGAGCACG | This study |
| 2007 | 5′-CTTGAATGCCCTGCAGATCTTGGCCGCTACAGATACGACACb | This study |
| 2008 | 5′-AAGATCTGCAGGGCATTCAAG | This study |
| 2009 | 5′-GGTCATCGGGGACGAGTG | This study |
| 2010 | 5′-CGAATAGCGGATGAGATCG | This study |
| 2011 | 5′-GTTGTCTCGAGCATGAGCAAGGATTTTCTGACCGCTTACG | This study |
| 2012 | 5′-TTGCTCATGCTCGAGACAAC | This study |
| 2013 | 5′-AATGGGCGATACGGTAACAG | This study |
| 2014 | 5′-ACGAGCTTCCGAAATACACG | This study |
| 2015 | 5′-ATCGAGACGATTCGTTCAGC | This study |
| 2016 | 5′-CGAGCATCTCAAAATTCATCC | This study |
| 2017 | 5′-CGTGTATTTCGGAAGCTCGTTAATGGGCGATACGGTAACAG | This study |
| 2018 | 5′- ATGAATCACGACCCGAACTG | This study |
| 2019 | 5′- CTTGCTCTCGTCCTCTTCCTACGATCAGCAACACGATCAG | This study |
| 2020 | 5′- AGGAAGAGGACGAGAGCAAG | This study |
| 2021 | 5′- GACGAAGCTGCTGAACGTC | This study |
| 2041 | 5′-AGATACGGCATCGGATTGAC | This study |
| 2042 | 5′-GTCGCCGGCTGATTATTTC | This study |
| 2043 | 5′-GCAACGCTTGTTTCAATACG | This study |
| 2044 | 5′-GAAAGGCTCGGTCACGTTC | This study |
| 2045 | 5′-AATTCGTCACACGAACATGC | This study |
| 2046 | 5′-CGTCATTCCACCTTCCATTG | This study |
| 2047 | 5′-AGGAGGTCTACCACCTGCAC | This study |
| 2048 | 5′-TTTTGTTTGCCGCCATTC | This study |
| 2187 | 5′-CGAGCTTTCGCTGTCCTATC | This study |
| 2188 | 5′-CGTGATCTTCGTGTCCTTGAGTTGTGTCATTGCGCTTCTC | This study |
| 2189 | 5′-TCAAGGACACGAAGATCACG | This study |
| 2190 | 5′-CCGGCAATTGATCGAACTC | This study |
| 2191 | 5′-CGATCAACGTGATCTTCGTG | This study |
| MUTAGENIC PRIMERS | ||
| 2022 | 5′Phos/-GAATCATTCTCCGTTG AAG CGCTCGCTGCTCGTCGCAGC | This study |
| 2075 | 5′Phos/-GCTTTCCCGTTCT A CAGCACATCCAAGATGATGC | This study |
| 2076 | 5′Phos/-GAATCATTCTCCGTTGCGC GC CTCGCTGCTCGTCGCAGC | This study |
| 2136 | 5′Phos/-GCGCCGTGTTCAGCTCAG A CTCGCGGCGATCGAGC | This study |
| 2137 | 5′Phos/-AAAGCATCATCTTG A ATGTGCTGCAGAACTGG | This study |
| REAL TIME PCR PRIMERS | ||
| Bp23S-F | 5′-GTAGACCCGAAACCAGGTGA | Mima and Schweizer (2010) |
| Bp23S-R | 5′-CACCCCTATCCACAGCTCAT | Mima and Schweizer (2010) |
| 2077 | 5′-GTTCTGCAGCACATCCAAGA | This study |
| 2078 | 5′-CGGTGTTGTCGCTGTACTGA | This study |
| CLONING | ||
| 1687 | 5′-GGATCCGACGAGAGCTGATACGCTAG | This study |
| 1712 | 5′-AAGCTTATACCGGCATCGTTTCGCTG | This study |
| 2010 | 5′-AGGCTGGCTGTACTTGAACG | This study |
| 2011 | 5′-CGGGCGATATTCTGATGTC | This study |
| TN7 INTEGRATION CONFIRMATION | ||
| Tn7L | 5′-ATTAGCTTACGACGCTACACCC | Choi et al. (2005) |
| BPGLMS1 | 5′-GAGGAGTGGGCGTCGATCAAC | Choi et al. (2008) |
| BPGLMS2 | 5′-ACACGACGCAAGAGCGGAATC | Choi et al. (2008) |
| BPGLMS3 | 5′-CGGACAGGTTCGCGCCATGC | Choi et al. (2008) |
aBold indicates a newly generated restriction enzyme cleavage site.
bUnderline indicates overlapping sequence for SOEing PCR; a double underline indicates a ribosome binding site.
cItalics indicates introduced point mutations.
dPhos, 5′ phosphorylated oligonucleotide.