Table 4.
β-lactam susceptibilities of PenA, TAT-signal sequence, and TAT secretion apparatus mutants.
| MIC (μg/mL) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Straina | Mutation | Amoxicillin | Amoxicillin + clavulanic acid | Ampicillin | Piperacillin | Carbenicillin | Ceftazidime | Imipenem | Meropenem | BAL30072 |
| 1026b | None | ≥256b | 3 | 256 | 8 | 1024 | 3 | 1.5 | 0.75 | 0.065 |
| Bp82 | Nonec | ≥256 | 3 | 128 | 8 | 1024 | 3 | 1.5 | 0.75 | 0.031 |
| Bp319 | ΔpenA | 6 | 3 | 8–16 | 2 | 32 | 2 | 0.25 | 0.75 | 0.016 |
| Bp82.11 | ΔpenA | ND | 3 | ND | ND | ND | ND | ND | ND | ND |
| Bp82.21 | Tn7-penA + IPTG | ≥256 | 16 | 2048 | 64 | >4096 | 32 | 16 | 1.5 | 0.25 |
| Bp82.3 | PenA C69Y | 6 | 3 | 4–8 | 6–8 | 32–64 | ≥256 | 0.19 | 0.75 | 0.031 |
| Bp82.4 | PenA S72F | ≥256 | 12–16 | 64–128 | 8 | 512 | 3 | 1–2 | 0.75 | 0.031 |
| Bp82.5 | PenA P167S | 6 | 3 | 4–8 | 4 | 32–64 | 16–24 | 0.19 | 0.5–0.75 | 0.031 |
| Bp409 | ΔtatABC | 4–6 | 3 | 4–8 | 3 | 16–32 | 1.5 | 0.19 | 0.5 | 0.016 |
| Bp420 | ΔpenA ΔtatABC | 4 | 3 | 4 | 2 | 16 | 1 | 0.25 | 0.05 | 0.016 |
| Bp342 | PenA R7K | ≥256 | 3 | 128 | 8 | 1024 | 3 | 2 | 0.75 | 0.031 |
| Bp421 | PenA R8A | 8 | 3 | 8 | 2 | 32 | 1 | 0.25 | 0.5 | 0.031 |
aBp319, Bp342, Bp409, Bp420, and Bp421 were derived from 1026b; Bp82.11 and Bp82.21 were derived from Bp82.
b≥256, the detection limit is 256 μg/mL.
cNo penA or tat mutation but strain is a 1026b ΔpurM derivative.
ND, not done.