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. Author manuscript; available in PMC: 2011 Jul 5.
Published in final edited form as: J Immunol. 2010 Mar 31;184(9):4761–4769. doi: 10.4049/jimmunol.0904149

FIGURE 3.

FIGURE 3

Activation of cell surface CD74 upregulates CLL migration to the BM in a TAp63-dependent manner. A, CLL cells were incubated in the presence or absence of hLL1 for 24 h. Cell migration toward CXCL12 was analyzed for 3 h through a Transwell plate coated with fibronectin. The graph shows one experiment out of three advanced CLL patients tested. B, CLL cells were treated with siRNA for p63 or for a control gene, lacz, for 26 h. Cell migration toward CXCL12 was analyzed for 3 h in a Transwell plate coated with fibronectin. The graph shows one experiment, representative of three. C–E, Early- and advanced-stage CLL cells were incubated in the presence or absence of MIF for 24 h. C, Cell migration toward CXCL12 was analyzed for 3 h in a Transwell plate coated with fibronectin. The graph shows one experiment representative offive early and four advanced patients tested. D and E, Cells were stained with CFSE and injected i.v. into C57BL/6 mice for 3 h. BM (D) and spleen (E) populations were analyzed by FACS. The graph shows the results of six early and seven advanced CLL samples.