Figure 1.

In vivo expression of unmodified and chimeric adenoviruses when injected via different routes. BALB/c mice were injected with 1 × 10¹⁰ virus particles of either Ad5GL or Ad5/6GL by different routes. (a) Mice were imaged 24 hours later using a Lumazone imager: intranasal, 10-minute 1 × 1 binning, 1,315–1,389 gray values; intramuscular, 10-minute 2 × 2 binning, 712–1,300 gray values; intravenous, 1 minute 1 × 1 binning, 1,296–2,000 gray values. Shown are representative white light images overlaid with luciferase expression in pseudo-color. (b) Quantification of luciferase expression in mice injected via different routes after 24 hours. N = 5. *P < 0.05; ns = not significant. Means ± SEM.