Figure 2.

Ad5/6GL and Ad5GL interact with blood factors comparably. (a) CHO, (b) CHO cells transiently transfected with coxsackie-adenovirus receptor (CAR), or (c) SKOV3 were infected with unmodified or chimeric adenovirus (Ad) vectors (multiplicity of infection 1,000) ± FX (10 µg/ml). A luciferase assay was performed after 24 hours to determine transgene expression. N = 6. (d) BALB/c mice were injected subcutaneously with warfarin (133 µg in 100 µl peanut oil) 3 and 1 day before intravenous injection of 1 × 10¹⁰ virus particles (vp) unmodified or chimeric adenoviruses. Mice were imaged 24 hours later using a Lumazone imager and quantified for luciferase transgene expression; 3 minutes 2 × 2 binning. N = 5. *P < 0.05; **P < 0.005; ns = not significant. Means ± SEM.