Figure 4.

Ad5/6 depletes Kupffer cells less than Ad5. BALB/c mice were injected intravenous (i.v.) with phosphate-buffered saline, a low dose [1 × 10¹⁰ virus particles (vp)], or a high dose (3 × 10¹⁰ vp) of adenovirus (Ad) vectors. Six hours later, the large lobe of the liver was harvested, paraffin embedded and sectioned (4 µm thick). (a,c–f) Immunohistochemical staining of Kupffer cells (anti-F4/80 macrophage marker). (b) Quantification of F4/80 positive cells 6 hours after i.v. injection of Ad vectors. **P < 0.005; ns = not significant. Means ± SEM.