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. Author manuscript; available in PMC: 2012 May 27.
Published in final edited form as: Immunity. 2011 May 19;34(5):755–768. doi: 10.1016/j.immuni.2011.02.020

Figure 4. Inflammation in KI mice is induced by IL-1β.

Figure 4

(A) Luminex analysis of serum cytokines of 8-week-old WT, MefvV726A/+, MefvV726A/V726A Rag1−/−, and MefvV726A/V726A Rag1−/− mice. *, P < 0.05; **, P < 0.0001; ***, P < 0.000001. (B) CD11b+ cells from BM of WT, MefvV726A/+, and MefvV726A/V726A mice were stimulated with LPS or no stimulus for 3 hr followed by treatment with or without ATP. Cell culture supernatants (Sup.) and cell lysates (Lys.) were analyzed by immunoblotting. (C–E) Analyses of MefvV726A/V726A mice on the Il1r1−/− background. (C) Body weights of 8-week-old males. (D) Flow cytometry analyses of peripheral blood cells from 8-week-old mice. Data are representative of three independent experiments. (E) Inflammasome activation in MefvV726A/V726A mice withIl1r1−/− background. Culture supernatants and lysates from BM CD11b+ cells were collected and subjected to immunoblot as described in (B). Additional information is provided in Figure S3.