FIG. 1.

Detection of 16S rRNA, gltA, and ompA genes by PCR amplification of Ixodes pacificus tick extracts. Ninety-six ticks were collected in the Napa Valley, California. Tick DNA was extracted, pooled, and used as template for PCR amplification. The PCR products were electrophoresed and observed by staining with ethidium bromide. kb, 1 kb DNA ladder (Promega). Ip, I. pacificus tick extract; (+), Rickettsia conorii DNA positive control; (−), no template negative control; arrow, PCR amplicons. PCR, polymerase chain reaction.