Phylogenetic trees of ompA (A) and gltA (B) genes of the two Rickettsia phylotypes in I. pacificus. Other selected sequences on the phylogenetic tree of the ompA gene are Rickettsia sp. CAFOP07-3 (EU544297.1), Ixodes scapularis endosymbiont (AB002268.1), Rickettsia aeschlimannii (DQ379981.1), Rickettsia massiliae (DQ212707.1), Rickettsia rhipicephali (EU109177.1), Rickettsia australis (AF149108.1), Rickettsia montanensis (AF045223.1), Rickettsia sibirica (AABW01000001.1), Rickettsia rickettsii (AY319293.1), Rickettsia slovaca (EU622810.1), Rickettsia honei (AF018075.1), Rickettsia africae (EU622980.1), Rickettsia parkeri (EU715288.1), Rickettsia heilongjiangii (AF179362.2), Rickettsia conorii (U43794.1), Rickettsia felis (AY727036.1), Rickettsia peacockii (AY319292.1), Rickettsia japonica (U83442.1), and Rickettsia akari (L01461.1). Other selected sequences on the phylogenetic tree of the gltA gene are I. scapularis endosymbiont (EF662058.1), Rickettsia felis (CP000053.1), Rickettsia helvetica (DQ821857.1), Rickettsia akari (U59717.1), Rickettsia canadensis (BAF79997.1), Rickettsia australis (U59718.1), Rickettsia africae (CP001612.1), Rickettsia japonica (AY743327.1), Rickettsia parkeri (EF102236.1), Rickettsia slovaca (AY129301.1), Rickettsia montanensis (U74756.1), Rickettsia honei (U59726.1), Rickettsia sibirica (DQ124930.1), Rickettsia typhi (U59714.1), Rickettsia heilongjiangensis (AY285776.1), Rickettsia bellii (NC_007940.1), Rickettsia prowazekii (NC_000963.1), Rickettsia rickettsii (DQ150689.1), Rickettsia conorii (NC_003103.1), Rickettsia aeschlimannii (DQ235776.1), Rickettsia massiliae (U59719.1), Rickettsia rhipicephali (U59721.1), and Rickettsia peacockii (DQ100162.1). The tree is inferred from the comparison of either ompA or gltA sequences by the neighbor-joining method using PHYLO_WIN. The numbers at nodes are the bootstrap values obtained from 1000 replicates. Bootstrap values >50 are shown at the nodes. Bootstrap values determined by the neighbor-joining method and maximum-parsimony are shown above the line and below the line, respectively. Bar, nucleotide distance.