Skip to main content
. Author manuscript; available in PMC: 2011 Jul 5.
Published in final edited form as: Stem Cells. 2010 Jul;28(7):1281–1291. doi: 10.1002/stem.447

Figure 1.

Figure 1

Embryonic stem cells lacking calreticulin harbor significant transcriptome shifts. (A): Field-emission scanning electron microscopy, of WT and crt−/− embryonic stem cells, shows no significant morphological differences. Scale bar, 10 µm. Inset: Reverse transcription-polymerase chain reaction confirms absence of crt in knockout stem cells (left, WT; right, crt−/−). (B): Volcano plot analysis of total RNA changes between WT and crt−/− embryonic stem cells revealed 2,904 significant down- and upregulated genes (blue), distinct from genes falling below significance threshold or minimum 1.5-fold change cutoff (gray). (C): Sample reproducibility allowed construction of an expression heatmap of 1,605 quality restricted genes (upper panel). Composition of the shifted transcriptome was 74% downregulated (green) and 26% upregulated (red) (lower panel). Abbreviation: WT, wild type.