Figure 3.

Loss of calreticulin impacts calcium handling processes. (A): Down- and upregulated calcium handling genes within a calreticulin-deficient background integrate into a heatmap and display pairwise correlation of gene expression changes. Color scale indicates range of fold change. (B): Calmyrin (Cib1) and cadherin 2 (Cdh2) as random examples of microarray-identified fold changes confirmed by reverse tran-scription-polymerase chain reaction. (C): Discrete sarcoglycan module (dashed box) and main network with highly connected hubs (blue circles) integrates downregulated (green) and upregulated (red) genes that together visualizes organization of transcripts affected by calreticulin knockout associated with calcium handling. Abbreviations: WT, wild type; TNF, tumor necrosis factor.