Figure 5.

Molecular attenuation of calreticulin impacts a transcriptome network with repercussions on cardiovascular development. (A): Onto-logical analysis of genes affected by calreticulin deficiency reveals transcripts that impact global processes of gene expression, protein trafficking, and molecular transport, with numerous accessory functions. (B): Re-analysis of the calreticulin-deficient transcriptome according to physiological development prioritized 11 distinct systems. (C): Genes associated with cardiac differentiation organized into a network of curated relationships within a calreticulin-deficient background. Composed of 45 network nodes, nine displayed increases in expression (red), while the remaining genes were downregulated (green), after calreticulin deletion. Within this network, Ccnd1 Ccnd2, and Notch1 are identified as network hubs (purple boxes), defined as nodes that possess the greatest number of connections to neighboring genes. Orange circles highlight genes with characterized effects on cardiac formation.