Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Principle of allele-specific primer elongation on microarrays. Single-stranded DNA targets serve as templates in a Taq polymerase-catalyzed elongation reaction of immobilized oligonucleotide primers. The match and mismatch primers differ at their free 3′-end by a variable base, which is discriminated by the enzyme. Elongation and thereby incorporation of Cy3-dUTP takes place in a template-dependent manner.
