FIG. 7.

Comparison of outgrowth on dAM generated by collagenase-isolated clusters and dispase-isolated sheets. Immunostaining confirmed universal expression of ABCG2 in cell membranes and p63α and Pax6 expression in nuclei of small basal epithelial cells, whereas CK12 was expressed by few large suprabasal cells (A). Single cells derived from the outgrowth isolated from dAM by dispase followed by T/E gave significantly more clones comprising all three classes of holoclones, meroclones, and paraclones from collagenase-isolated clusters when compared to dispase-isolated sheets (B, rhodamin B staining on day 9, *p < 0.05, **p < 0.001). Scale bar = 100 μm. Color images available online at www.liebertonline.com/tec