Figure 5.

Vector dose–response profile. Effect of vector dose on luciferase transgene expression levels in (a) heart and (b) liver. Parental adeno-associated virus serotype 9 (AAV9) and AAV9.45 vectors were administered intravenously at low (1 × 10¹⁰ vg/mouse), medium (5 × 10¹⁰ vg/mouse) and high (1 × 10¹¹ vg/mouse) dose. Luciferase expression assays were carried out to at 2 weeks postadministration and normalized for total tissue protein concentration. Data are represented as relative light units and all experiments were carried out in triplicate. Error bars represent standard deviation.