Figure 1.

Starting genomic segment and constructs required for assembly. (A) The 90 kb P.rosea genomic fragment and the three cosmids pRP16, pRP31 and pRP58. Fragments A–D are color-coded. (B) The in vitro generated plasmids pMAB1, pMBC1, pMCD1 and pPAD1. (C) Scheme of cointegrate formation and resolution between each cosmid and the cognate ts construct. The interrupted empty bars designate the genomic segments comprised between fragments A and B, B and C, and C and D. Chloramphenicol and kanamycin are abbreviated to Cm and Km, respectively. cat, aph and tet represent Cm, Km and tetracycline resistance genes, respectively; ts, the temperature-sensitive replication origin. DraI sites in pPAD1 are indicated as D.