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. 2001 Apr 1;29(7):e37. doi: 10.1093/nar/29.7.e37

Figure 3.

Figure 3

Isolation and resolution of a cointegrate. (A) Selection for the pMCD1::pRP58 cointegrate at 44°C (non-permissive temperature) and resolution at 30°C to yield pMCD2. The antibiotics used for selection are indicated. BamHI sites are indicated by bars. Other symbols and abbreviations are as in Figure 1. (B) Analysis of cointegrates and resolved cointegrates. The BamHI profiles of pMCD2 (lane 1), of three independent pMCD1::pRP58 cointegrates (lanes 2–4) and of pRP58 (lanes 5–6) are shown. M, molecular weight marker, with relevant sizes (in kb) on the left.