Figure 4.

Adeno-associated virus serotype 9 (AAV9) transduces a variety of cell types after i.v. delivery to the mouse central nervous system (CNS). (a–f) Alternate serial sections described in Figure 1 were subjected to co-immunofluorescence with the indicated antibodies. For each set of three panels, the left panel shows green fluorescent protein (GFP) expression, the middle shows labeling with the indicated antibody, and the right panel shows a merged image. The inset in the right panel highlights co-localization observed, and the arrow points to the cells magnified. All images are four merged confocal Z-stacks of samples from mice injected with 2 × 10¹¹ vg (1 × 10¹³ vg/kg), except for panel f which shows the 1.6 × 10¹² vg (8 × 10¹³ vg/kg) dose. Note that with panel e, native fluorescence was used to visualize GFP expression. Scale bars for all panels are shown in the right panels. Bar = 200 µm.