Figure 5.

Mannitol and vehicle can modestly affect adeno-associated virus serotype 9 (AAV9) mouse central nervous system (CNS) delivery. In each case, mice received a tail vein injection of 1×10¹¹ vg (5×10¹² vg/kg) of scAAV9/GFP. After 4 weeks, tissues were harvested, and extracted DNA tested by quantitative PCR as described in the methods. (a) Mice received an intravenous (i.v.) injection of mannitol 8 minutes prior to i.v. vector injection. (b) A virus stock was diluted to create the vehicle compositions shown, then intravenously injected into mice. Error bars indicate standard deviation. n.d., no data. *P < 0.05 compared to 167 mmol/l phosphate-buffered saline + 0.7% sorbitol (Student's T-test: two-tailed, unpaired, equal variance).