Figure 8.

Intravascular adeno-associated virus serotype 9 (AAV9) delivery in nonhuman primates (NHPs) transduces mostly glia in the central nervous system (CNS). Representative sections from the NHPs showing higher levels of transduction (left panels: i.c., 26149; right panels: i.v., 26945) were selected and subjected to co-immunofluorescence with the indicated antibodies. For each picture, green fluorescent protein-expressing cells are green and (a–f) glial fibrillary acidic protein or (g–l) NeuN-positive cells are red. Control sections in which no primary antibodies were used are depicted in (m) green and (n) merged green and red. The insets highlight observed co-localization, arrows pointing to the magnified cells. Scale bars for all panels are on the bottom panels. Bar = 100 µm.