Figure 6.
MicroRNA (miRNA)-regulated vaccinia virus (MRVV) luciferase (L) and enhanced green fluorescent protein (G) reporters (MRVV/LG) reduces viral pathogenicity while maintaining oncolytic activity in vivo. (a,c) Nude mice bearing established subcutaneous (a) BxPC-3 tumors or (c) A549 tumors were treated with intratumoral injections of MRVV/LG [1 × 107 plaque-forming unit (pfu)/injection, 3 × 107 pfu/mouse] on days 0, 3, and 6. The vaccinia virus designations are the same as those used in Figure 4a. The data are presented as mean ± SD (n = 5). (b,d) Survival curves of the mice that are shown in a and c. (e) In vivo biodistribution of MRVV/LG, determined by noninvasive imaging after intraperitoneal injection of -luciferin into the mice that are shown in (a, b) on days 27 and 52.